HPLC Analysis of Chinese Ginseng Extract on Ascentis® Express C18

HPLC Analysis of Chinese Ginseng Extract on Ascentis® Express C18

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles (53829-U)
column temp. 60 °C
mobile phase [A] water; [B] acetonitrile
gradient held at 25% B for 1.5 min; 25 to 85% B in 12 min; held at 85% for 1 min
flow rate 1 mL/min
pressure 3481 psi (240 bar)
sample Extracted from dried, ground plant material; 10 mg/mL in water:acetonitrile (85:15); sonicate 10 min at ambient
injection 25 µL
detector UV, 205 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of Chinese ginseng root extract.
Categories Analytical Chromatography, Herbal medicines
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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