HPLC Analysis of Chloramphenicol in Milk on Ascentis® C18 after SPE using SupelMIP® SPE-Chloramphenicol

HPLC Analysis of Chloramphenicol in Milk on Ascentis® C18 after SPE using SupelMIP® SPE-Chloramphenicol

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix whole pasteurized milk spiked with chloramphenicol at 15 ng/mL and 38 ng/mL
SPE tube/cartridge SupelMIP SPE - Chloramphenicol, 25 mg/10mL (LRC) (53210-U)
condition 1 mL methanol;1 mL DI water
sample addition 1 mL
washing 2 x 1 mL MS-grade water, 1 mL 5% acetonitrile in 0.5% acetic acid, 2 x 1 mL MS-grade water, 1 mL 20% acetonitrile in 1% ammonium hydroxide. (dry SPE tubes for 15 min. under gentle vacuum; 3 x 1 mL dichloromethane; dry SPE tubes for 1 min. under gentle vacuum)
elution elute chloramphenicol with 2 x 1 mL methanol:acetic acid:MS-grade water (89:1:10, v/v/v)
eluate post-treatment evaporate combined eluate to dryness at 50 °C under nitrogen. reconstitute in 150 mL LC mobile phase prior to LC-MS analysis
column Ascentis C18, 2.1 mm x 10 cm I.D., 3 µm particles (581301-U)
column temp. 35 °C
mobile phase [A] 100 mM ammonium acetate: [B] MS-grade water: [C] acetonitrile (10:60:30, A:B:C)
flow rate 0.2 mL/min
injection 5 µL
detector MS, ESI(-), 320-323 m/z range

Description

Categories Analytical Chromatography, SPE Applications, Food & Beverage Analysis, Pharmaceutical & Clinical applications, Beverage Testing, Veterinary Drug Residues, Antibiotics/antimicrobials, Milk, Applications (Chromatograms)
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, SupelMIP is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for SPE

Materials

     
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