HPLC Analysis of Chlorhexidine and Robenidine on Ascentis® Phenyl

HPLC Analysis of Chlorhexidine and Robenidine on Ascentis® Phenyl

Conditions

column Ascentis Phenyl 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; [C] 500 mM ammonium formate, pH 3.0 with formic acid
gradient 10% C constant; 65% A, 25% B to 0% A, 90% B in 7 min; held for 2 min; to 65% A, 25% B in 0.5 min
flow rate 1.0 ml/min
sample 100 ug/mL each in 70:30, 25 mM ammonium formate pH 3.0:acetonitrile
injection 10 µl
detector UV, 273 nm

Description

Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Cosmetics, Personal Care, and Cleaning Products
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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