HPLC Analysis of Cold Remedy Ingredients on Ascentis® RP-Amide

HPLC Analysis of Cold Remedy Ingredients on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 30 C
mobile phase [A] 5 mM ammonium formate, pH 2.75 with formic acid; [B] acetonitrile
gradient 10 to 30% B in 15 min; held for 2 min; to 10% B in 0.1 min
flow rate 1.0 ml/min
sample as indicated in 5 mM ammonium formate (pH 2.75 with formic acid)
injection 10 µl
detector UV, 270 nm

Description

Categories Analytical Chromatography, Antihistamines
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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