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HPLC Analysis of Column Test Mixture on Ascentis® Express RP-Amide

HPLC Analysis of Column Test Mixture on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RPA 15 cm x 4.6 mm, 5 µm particles (50773-U)
column temp. 26 C
mobile phase [A] 20 mM potassium phosphate, pH 7; [B] acetonitrile; (70:30, A:B)
flow rate 4.0 ml/min
pressure 4467 psi (308 bar)
sample 1. Uracil (0.016 mg/mL), 2. Benzamide (0.161 mg/mL), 3. Aniline (0.161 mg/mL), 4. Cinnamyl Alcohol (0.016 mg/mL), 5. Dimethyl Phthalate (0.323 mg/mL), (6. 2-Nitroaniline (0.081 mg/mL), 7. 4'-Bromoacetanilide (0.040 mg/mL), 8. 2,2'-Biphenol (0.161 mg/mL), 9. 4,4'-Biphenol (0.040 mg/mL), in 50/50 Water/Acetonitrile)
injection 5 µl
detector UV, 254 nm

Description

Analysis Note This application shows a test mixture of compounds of widely varying polarity on the 5μm RPA Ascentis Express column.
Featured Industry General Analytical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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