HPLC Analysis of Conjugated Estrogens on Discovery® HS C18

HPLC Analysis of Conjugated Estrogens on Discovery® HS C18

Conditions

column Discovery HS C18, 15 cm x 4.6 mm I.D., 5 µm particles (568522-U)
column temp. 30 °C
mobile phase [A] 10 mM potassium phosphate (pH 7.01 unadjusted); [B] acetonitrile; [C] methanol
gradient 80% A, 15% B, 5% C, held for 5 min; to 40% A, 45% B, 15% C in 20 min; held for 5 min
flow rate 1 mL/min
sample 10 µg/mL each in 60/40, water:acetonitrile
injection 10 µL
detector UV, 220 nm

Description

Categories Analytical Chromatography, Clinical, Hormones, proteins
Featured Industry Clinical
Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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