HPLC Analysis of Cortocisteroids in Urine on Discovery® HS F5 after SPE using Discovery® DSC-CN

HPLC Analysis of Cortocisteroids in Urine on Discovery® HS F5 after SPE using Discovery® DSC-CN

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix human urine spiked with corticosteroids at 0.5 and 1.0 µg/mL and diluted in DI water (1:1, v/v)
SPE well plate Discovery DSC-CN 96-well SPE, 100 mg/well (575636-U)
condition 1 mL methanol; 1 mL DI water.
sample addition 1 mL spiked urine
washing 1 mL 20% methanol
elution 1 mL methanol
eluate post-treatment Evaporate eluate with nitrogen at 30 °C. Reconstitute in 200 µL mobile phase.
column Discovery HS F5, 5 cm x 4.6 mm I.D., 3 µm particles (567504-U)
column temp. 35 °C
mobile phase [A] methanol; [B] DI water; (40:60, A:B)
flow rate 1.5 mL/min
injection 5 µL
detector UV, 240 nm

Description

Categories Analytical Chromatography, Clinical, SPE Applications, Steroids, steroids, Pharmaceutical & Clinical applications
Featured Industry Clinical
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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