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HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 µm particles (568222-U)
column temp. ambient
mobile phase [A] (0.1% TFA in water):(0.1% TFA in acetonitrile); (70:30), [B] (0.1% TFA in water):(0.1% TFA in acetonitrile), 64:36
gradient 0-100% B in 30 min
flow rate 1 ml/min
injection 12 muLeach at 0.8 mg/mL in 0.1%TFA
detector UV, 220 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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