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HPLC Analysis of Cytochrome c Tryptic Digest on Discovery® C18

HPLC Analysis of Cytochrome c Tryptic Digest on Discovery® C18

Conditions

column Discovery C18, 15cm x 4.6mm I.D., 5mum particles (504955)
column temp. 35 C
mobile phase [A] 0.1% TFA, 3% n-propanol in water; [B] 0.1% TFA, 3% n-propanol in acetonitrile
gradient 1-37% B in 72 min
flow rate 1 ml/min
detector UV, 220 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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