HPLC Analysis of Deoxyribonucleosides and Ribonucleosides on SUPELCOSIL™ LC-18-S

HPLC Analysis of Deoxyribonucleosides and Ribonucleosides on SUPELCOSIL™ LC-18-S

Conditions

column SUPELCOSIL LC-18-S, 15 cm x 4.6 mm I.D., 5 µm particles (58931)
column temp. 30 C
mobile phase 0.05 M dipotassium phosphate/monobasic potassium phosphate, pH 4.0:methanol [A] 97.5:2.5; [B] 80:20
flow rate 1 ml/min
sample nucleoside standards in water
detector UV, 254 nm

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Life Science and Biopharma
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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