HPLC Analysis of Derivatized Diisocyanates on Ascentis® Express RP-Amide

HPLC Analysis of Derivatized Diisocyanates on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-AMIDE 15 cm x 4.6 mm, 2.7 µm particles (53931-U)
column temp. 26 C
mobile phase [A] 50mM Ammonium Acetate; [B] Acetonitrile
gradient 30% B held for 1.5 min; 30 to 60% B in 10 min; held at 60% B for 5 min
flow rate 0.8 ml/min for 1.5 min; 0.8 ml/min to 1.5 ml/min in 10 min.
sample 125 µg/mL 1-2PP + 10 µg/mL 2,6-TDIP + 10 µg/mL 1,6-HDIP + 10 µg/mL 2,4-TDIP + 10 µg/mL 4,4-MDIP in 70:30 Water:ACN
injection 5 µl
detector UV, 254 nm

Description

Analysis Note This application shows the separation of four common derivatized diisocyanate compounds related to the use of ORBO80 filters on fused core technology.
Categories Analytical Chromatography, Air Monitoring Applications, Isocyanates
Featured Industry Environmental
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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