HPLC Analysis of Diclofenac and Salicylic Acid on Ascentis® RP-Amide

HPLC Analysis of Diclofenac and Salicylic Acid on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase [A] 20 mM phosphoric acid (pH 2.0 unadjusted); [B] acetonitrile; (50:50, A:B)
flow rate 1.0 ml/min
sample as indicated in 20 mM phosphoric acid (pH 2.0)
injection 10 µl
detector UV, 220 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Anti-inflammatories
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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