HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express C18

HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express C18

Conditions

column Ascentis Express C18, 3 cm x 4.6 mm I.D., 2.7 µm particles (53818-U)
column temp. 40 C
mobile phase [A] 100 mM ammonium acetate, pH 5.6 (titrated with acetic acid); [B] water; [C] acetonitrile
gradient 20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
flow rate 3 ml/min
pressure 3191 psi (220 bar)
sample neotame 350 µg/mL in diet cola
injection 1 µl
detector UV, 214 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.
Categories Analytical Chromatography, Food & Beverage Analysis, Flavors, Fragrances, Beverage Testing, Soft Drinks
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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