HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express RP-Amide

HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 3 cm x 4.6 mm I.D., 2.7 µm particles (53921-U)
column temp. 40 °C
mobile phase [A] 100 mM ammonium acetate, pH 5.6 with acetic acid; [B] water; [C] acetonitrile
gradient 20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
flow rate 3 mL/min
pressure 3336 psi (230 bar)
sample diet soda spiked with neotame 350 µg/mL
injection 1 mL/min
detector UV, 214 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in diet cola.
Categories Analytical Chromatography, Flavors, Fragrances
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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