HPLC Analysis of Dopamine and Related Compounds on Discovery® HS

HPLC Analysis of Dopamine and Related Compounds on Discovery® HS

Conditions

column Discovery HS F5, 15 cm x 4.6 mm I.D., 5 µm particles (567516-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate (pH 3.0 with concentrated formic acid), [B] acetonitrile
flow rate 1 mL/min, split to the MS
sample 50 µg/mL in 10 mM ammonium formate (pH 3.0 with formic acid)
injection 25 µL
detector MS in SIR (Selected Ion Recording) mode using (+) or (-) ESI mode

Description

Categories Analytical Chromatography, Clinical, Hormones, Catecholamines, Neurotransmitters, catecholamines, neurotransmitters, antidepressants, Antidepressants, hormones
Featured Industry Clinical
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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