HPLC Analysis of E. coli Lysate on Proteomix® SAX-NP3, Demonstration of High Loading Capacity

HPLC Analysis of E. coli Lysate on Proteomix® SAX-NP3, Demonstration of High Loading Capacity

Conditions

column Proteomix SAX-NP3, 5 cm x 4.6mm I.D., 3 µm particles (Z777219)
column temp. 25 C
mobile phase (A) 20 mM Tris, pH 9.0; (B) A + 0.5 M NaCl
gradient 0 - 100% B in 30 min
flow rate 0.5 ml/min
sample 2.5 mg/mL
injection as indicated in figure
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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