HPLC Analysis of Erectile Dysfunction Drugs on Ascentis® Express Phenyl-Hexyl

HPLC Analysis of Erectile Dysfunction Drugs on Ascentis® Express Phenyl-Hexyl

Conditions

column Ascentis Express Phenyl-Hexyl, 10 cm x 3.0 mm I.D., 2.7 µm particles (53345-U)
column temp. 35 °C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient 5% B held for 0.5 min; to 100% B in 9.5 min; 100% B held for 2 min
flow rate 0.4 mL/min
pressure 2030 psi (140 bar)
sample 50 µg/mL in 85:15, water:methanol
injection 2 µL
detector UV, 250 nm

Description

Categories Analytical Chromatography, ED Drugs
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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