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HPLC Analysis of Erectile Dysfunction Drugs on Ascentis® Express RP-Amide

HPLC Analysis of Erectile Dysfunction Drugs on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 5 µm particles (50753-U)
column temp. 35 C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient 5% B held for 0.5 min; to 100% B in 9.5 min; 100% B held for 2 min
flow rate 0.4 ml/min
pressure 870 psi (60 bar)
sample 20 µg/mL in water:methanol (85:15)
injection 2 µl
detector UV, 250 nm

Description

Categories Analytical Chromatography, ED Drugs
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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