HPLC Analysis of Erythropoietin on SRT® SEC-300 Gel Filtration Column

HPLC Analysis of Erythropoietin on SRT® SEC-300 Gel Filtration Column

Conditions

column SRT SEC-300, 30 cm x 7.8 mm I.D., 5 µm partices, 300 Ã…, (Z777051)
column temp. Ambient
mobile phase Na2HPO4-KH2PO4-NaCl
flow rate 0.7 ml/min
sample EPO (Recombinant Human Erythropoietin)
injection 20 µl
detector UV, 280 nm

Description

Analysis Note Erythropoietin (EPO) is a glycoprotein hormone that controls red blood cell production. EPO was one of the first genetically engineered therapeutic proteins. It is available under such brand names as Epogen and Aranesp for the treatment of anemia. Human EPO has a molecular mass of 34 kDa. The 5 micron SRT SEC-300 column is operated at a flow rate of 0.7 ml/min. Note that after the main peak, an impurity elutes at about 16 minutes.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information SRT is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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