HPLC Analysis of Estrogens on Ascentis® Express Biphenyl

HPLC Analysis of Estrogens on Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 10 cm x 2.1 mm I.D., 2.7 µm particles (64065-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile, (60:40, A:B)
flow rate 0.4 ml/min
pressure 2900 psi (200 bar)
sample 60 µg/mL each in 50:50, water:organic solvent
injection 2 µl
detector UV, 220 nm

Description

Categories Analytical Chromatography, Steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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