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HPLC Analysis of Fab/Fc mAb Antibody Fragments on Proteomix® WCX NP1.7

HPLC Analysis of Fab/Fc mAb Antibody Fragments on Proteomix® WCX NP1.7

Conditions

column Proteomix WCX-NP1.7, 3 cm x 4.6 mm I.D., 1.7 µm particles (Z777179)
mobile phase (A) 20 mM sodium acetate, pH 5.15; (B) A + 1 M LiCl
gradient 12% to 40% B in 6.7 min; to 100% B in 0.1 min; held for 2 min
flow rate (1.5 ml/min)
pressure 3335-3916 psi (230 - 270 bar)
sample 1 mg/mL papain digested MAb 321
injection 25 µl
detector UV 280 nm

Description

Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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