Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si, 15 cm Column

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si, 15 cm Column

Conditions

column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 30 C
mobile phase [A] hexane, [B] ethyl acetate
gradient 10 to 30% B in 10 min; held for 2 min; to 10% B in 1 min
flow rate 1.0 ml/min
sample as indicated in 96:4 (hexane:isopropanol)
injection 10 µl
detector UV, 290 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links