HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase

HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles (53829-U)
column temp. 30 °C
mobile phase methanol
flow rate 1.5 mL/min
pressure 3249 psi (224 bar)
sample 0.5 mg/mL tocopherol acetate, alpha-tocopherol, delta-tocopherol and K1, 0.05 mg/mL D2, D3 and retinol acetate, 1.5 mg/mL retinol palmitate, all in ethanol.
injection 5 µL
detector UV, 280 nm

Description

Analysis Note Analysis was conducted using standard solution, detection via UV.
Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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