HPLC Analysis of Five Antibodies TSKgel® CM-STAT vs. Competitive Non-porous Cation Exchange Column

HPLC Analysis of Five Antibodies TSKgel® CM-STAT vs. Competitive Non-porous Cation Exchange Column

Conditions

column TSKgel CM-STAT, 10 cm x 4.6 mm I.D., 7 µm particles (821966)
column temp. ambient
mobile phase [A] 20 mM MES, pH 6.0; [B] 20 mM MES, 0.5 M NaCl, pH 6.0 (821966)
gradient 10 to 30% B in 15 min; step to 100% B; held for 2 min; step to 10% B; held for 4 min (competive column: 10 to 30% B in 30 min; step to 100%; held for 2 min; step to 10% B; held for 4 min)
flow rate 1 ml/min (competive column: 2 ml/min)
injection 20 µl
detector UV, 280 nm

Description

Categories Analytical Chromatography, Antibodies
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
Related Links