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HPLC Analysis of FMOC-Proline Enantiomers on Astec® CYCLOBOND® I 2000 SN

HPLC Analysis of FMOC-Proline Enantiomers on Astec® CYCLOBOND® I 2000 SN

Conditions

column CYCLOBOND I 2000 SN, 25 cm x 4.6 mm I.D., 5 µm particles (20524AST)
column temp. 25 C
mobile phase [A] acetonitrile; [B] methanol; [C] acetic acid; [D] ammonium hydroxide; (85:15:0.4:0.1, A:B:C:D)
flow rate 0.9 ml/min
sample 2 mg/mL in methanol
injection 5 µl
detector UV, 254 nm

Description

Categories Analytical Chromatography, Chiral Applications, Amino Acids, Life Science & Biopharma, All Biochemical Applications, All Amino Acids, FMOC-Amino Acids
Featured Industry Life Science and Biopharma
Legal Information Astec is a registered trademark of Sigma-Aldrich Co. LLC, CYCLOBOND is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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