HPLC Analysis of Four Proteins on Zenix® SEC-300, Comparison of Column Internal Diameter

HPLC Analysis of Four Proteins on Zenix® SEC-300, Comparison of Column Internal Diameter

Conditions

column Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (Z777028)
column Zenix SEC-300, 30 cm x 2.1 mm I.D., 3 µm particles (Z777024)
column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles (Z777033)
column temp. 25 °C
mobile phase 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid
flow rate 15, 72, 207 µL/min (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
pressure 220, 240, 350 psi (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
sample (Waters SEC200 Protein Std)
injection 0.3, 1.4, 4.0 µL (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
detector UV, 215 nm

Description

Analysis Note Smaller column internal diameters (ID) are attractive for reasons of low solvent consumption. However, when adequate sample is available, larger ID analytical columns can be advantageous because they provide better column performance.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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