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HPLC Analysis of Furocoumarins on Ascentis® Express RP-Amide

HPLC Analysis of Furocoumarins on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 µm particles (53929-U)
column temp. 35 C
mobile phase [A] water; [B] methanol; (40:60, A:B)
flow rate 1.0 ml/min
pressure 2843 psi (196 bar)
sample 25 µg/mL in water:methanol (90:10)
injection 5 µl
detector UV, 254 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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