HPLC Analysis of Ginsenosides on Ascentis® Express C18

HPLC Analysis of Ginsenosides on Ascentis® Express C18

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles (53829-U)
column temp. 35 °C
mobile phase [A] water; [B] acetonitrile
gradient 25% B for 1.5 min; 25 to 85% B in 12 min; hold at 85% B for 1 min
flow rate 1 mL/min
sample 50 µg/mL in 82:18, water:methanol
injection 5 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Glycosides
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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