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HPLC Analysis of Glycan and Isomers on Proteomix® SAX-NP5, High Resolution Separation

HPLC Analysis of Glycan and Isomers on Proteomix® SAX-NP5, High Resolution Separation

Conditions

column Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 µm particles (Z777228)
mobile phase (A) 2.5% (v/v) acetic acid, 0.5% TEA in water; 0.5% acetic acid in acetonitrile
gradient 0-100% B in 60 min
flow rate 0.3 ml/min
sample 2-AA (anthranilic acid) labeled N-linked oligosaccharide profiling of an IgG1 sample
detector Fluorescence, Ex/Em=360/425 nm

Description

Categories Analytical Chromatography, Carbohydrates, Proteins
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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