HPLC Analysis of Heterocyclic Amines (HCAs) on Discovery® HS F5

HPLC Analysis of Heterocyclic Amines (HCAs) on Discovery® HS F5

Conditions

column Discovery HS F5, 15 cm x 4.6 mm I.D., 5 µm particles (567516-U)
column temp. 35 °C
mobile phase [A] water (10 mM ammonium acetate, pH 4.0 with acetic acid); [B] methanol (v/v); (15:85, A:B)
flow rate 1.0 mL/min
sample 50 µg/mL each in mobile phase
injection 5 µg/mL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Carcinogens, Amines
Featured Industry Chemicals and Industrial Polymers
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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