HPLC Analysis of HPLC Protein Standard on BIOshell™ A400 Protein C4

HPLC Analysis of HPLC Protein Standard on BIOshell™ A400 Protein C4

Conditions

column BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 µm particles (67463-U)
column temp. 30 C
mobile phase [A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75
gradient 0 to 75% B in 15 min
flow rate 0.3 ml/min
pressure 2800 psi (193 bar)
sample ribonuclease A, cytochrome c, holo-transferrin and myoglobin 1 mg in mobile phase A
injection 2 µl
detector UV, 215 nm

Description

Analysis Note Analysis of protein standard of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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