HPLC Analysis of HSA and HSA Fusion Protein on Zenix®-C SEC-300

HPLC Analysis of HSA and HSA Fusion Protein on Zenix®-C SEC-300

Conditions

column Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles (Z777092)
column temp. 25 °C
mobile phase (A) 150 mM sodium phosphate buffer, pH 7.0; (B) IPA; (95:5, v/v, A:B)
flow rate 1 mL/min
sample HSA fusion peptide 5 mg/mL (MW 75 kD, pl 5.0, HSA conjugated peptide in diabetes treatment)
injection 10 µL
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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