HPLC Analysis of HSA Fusion Protein on Proteomix® SAX-NP5

HPLC Analysis of HSA Fusion Protein on Proteomix® SAX-NP5

Conditions

column Proteomix SAX-NP5 25 cm x 4.6mm I.D., 5 µm particles (Z777229)
column temp. 30 °C
mobile phase Piperazine, Triethanolamine, bis-tris propane and N-methylpiperazine, 20 mM each,(A) pH 9.7; (B) pH 3.0 ( adjust by HCl )
flow rate 0.5 mL/min
pressure 2016 psi (139 bar)
sample HSA fusion peptide 5 mg/mL, 10 µL (MW 75 kD, pI 5.0, HSA conjugated peptide in diabetes treatment)
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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