HPLC Analysis of Human Growth Hormone (hGH) on Discovery® BIO PolyMA-WAX

HPLC Analysis of Human Growth Hormone (hGH) on Discovery® BIO PolyMA-WAX

Conditions

column Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm I.D., 5 µm particles (59602-U)
column temp. 25 C
mobile phase [A] 20 mM Tris-HCl, pH 8.0; [B] 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0
gradient 5 to 70% B in 30 min. (linear)
flow rate 0.5 ml/min
injection 10 mghGH
detector UV, 210 nm

Description

Analysis Note This Figure shows the degradation products of human growth hormone (hGH) well-separated on a Discovery BIO PolyMA-WAX anion-exchange column. The difference between the resolved compounds is the conversion of protein amide(s) to carboxylate(s), demonstrating both the power of the ion-exchange technique, and the efficiency of the Discovery BIO PolyMA-WAX column.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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