HPLC Analysis of Hydrocortisone in Topical Cream on Discovery® HS C18 after SPE using Discovery® DSC-Si

HPLC Analysis of Hydrocortisone in Topical Cream on Discovery® HS C18 after SPE using Discovery® DSC-Si

Conditions

sample preparation SPE (Solid Phase Extraction)
SPE tube/cartridge Discovery DSC-Si, 500 mg/3 mL (52695-U)
condition 1 mL hexane:ethyl acetate (2:1, v/v)
sample addition hydrocortisone topical hand cream in hexane:ethylacetate
sample addition 1 mL
washing 1 mL hexane:ethyl acetate (2:1, v/v)
elution 1 mL methanol:DI water (1:1, v/v)
eluate post-treatment dilute eluate to 2 mL with methanol:DI water (1:1, v/v)
column Discovery HS C18, 15 cm x 4.6 mm I.D., 5 µm particles (568520-U)
column temp. 35 C
mobile phase [A] methanol; [B] DI water (1:1, A:B)
flow rate 1 ml/min
injection 10 µl
detector UV, 220 nm

Description

Application Sample Pre-treatment:
Dissove 1 g 1% hydrocortisone topical hand cream in 10 mL ethyl acetate. Dilute 2 mL of ethyl acetate-cream sample with 8 mL hexane:ethyl acetate (2:1, v/v).
Categories Analytical Chromatography, SPE Applications, Steroids, Pharmaceutical & Clinical applications
Featured Industry Cosmetics, Personal Care, and Cleaning Products
Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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