HPLC Analysis of Iloperidone and Hydroxy Iloperidone Using a Titan™ C18 Column

HPLC Analysis of Iloperidone and Hydroxy Iloperidone Using a Titan™ C18 Column

Conditions

column Titan C18, 10 cm x 3.0 mm I.D., 1.9 µm particles (577160-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate; pH 3.0 with formic acid [B] 0.1% formic acid in methanol
gradient 45% B for 2 minutes followed by a linear gradient to 80% B for 10 minutes, followed by a hold at 80% B for 2 minutes.
flow rate 0.5 mL/min
pressure 6500 psi (445 bar)
sample 50 µg/mL and 5 µg/mL of hydroxy metabolite in 10% methanol
injection 2 µL
detector UV, 275 nm

Description

Analysis Note Iloperidone is an atypical antipsychotic administered for the treatment of schizophrenia. The drug is marketed under the trade names Fanapt® and Fanapta. Hydroxy iloperidone is a major metabolite of the atypical antipsychotic iloperidone which is administered for the treatment of schizophrenia. The metabolite is also pharmacologically active, possessing central nervous system (CNS) activity.
Categories Analytical Chromatography, Antidepressants
Featured Industry Pharmaceutical (small molecule)
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC, Fanapt is a registered trademark of Vanda Pharmaceuticals
suitability application for HPLC

Materials

     
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