HPLC Analysis of Insulin (Species Variants) on Discovery® BIO Wide Pore C8

HPLC Analysis of Insulin (Species Variants) on Discovery® BIO Wide Pore C8

Conditions

column Discovery BIO Wide Pore C8, 15 cm x 4.6 mm I.D., 5 µm particles (568322-U)
column temp. 30 °C
mobile phase [A] (0.1% TFA in water):(0.1% TFA in acetonitrile), 71:29 [B]: (0.1% TFA in water):(0.1% TFA in acetonitrile), 68:32,
gradient 0-100% B in 30 min
flow rate 1 mL/min
injection 5 µLin 0.1% TFA containing 5ug each of three insulin variants
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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