HPLC Analysis of Insulin (Species Variants) on Discovery® BIO Wide Pore C5

HPLC Analysis of Insulin (Species Variants) on Discovery® BIO Wide Pore C5

Conditions

column Discovery BIO Wide Pore C5, 15 cm x 4.6 mm I.D., 5 µm particles (568422-U)
column temp. ambient
mobile phase [A]: 71:29, (0.1% TFA in water):(0.1% TFA in acetonitrile); [B]: 68:32, (0.1% TFA in water):(0.1% TFA in acetonitrile)
gradient 0-100% B in 30 min
flow rate 1 ml/min
injection 5 µl5 µl in 0.1% TFA containing 5ug each of three insulin variants
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links