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HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide CN

HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide CN

Conditions

column BIOshell A160 Peptide CN, 15 cm x 2.1 mm, 2.7 µm particles (66969-U)
column temp. 75 C
mobile phase [A] 75:25 water (0.1% trifluoroacetic acid): acetonitrile (0.1% trifluoroacetic acid); [B] 50:50 water (0.1% trifluoroacetic acid): acetonitrile (0.1% trifluoroacetic acid); (87:13, A:B)
flow rate 0.5 ml/min
pressure 3200 psi (220 bar)
sample Mixture of six insulin variants, 100 µg/mL in 0.1% aqueous trifluoroacetic acid
injection 0.5 µl
detector UV, 215 nm

Description

Analysis Note The BIOshell A160 Peptide CN, with cyanopropyl functional groups, can provide alternative selectivity to a traditional C18 phase. Selectivity using the CN phase is primarily due to hydrophobicity and dipole-dipole interactions. A mixture of six insulin variants was separated using the BIOshell A160 Peptide CN column. Alternative selectivity of the first two analytes is seen using this column compared to the BIOshell A160 Peptide C18.
Categories Analytical Chromatography, Proteins, Peptides
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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