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HPLC Analysis of Isoflavones on Ascentis® Phenyl

HPLC Analysis of Isoflavones on Ascentis® Phenyl

Conditions

column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium formate (pH 3.0 with concentrated formic acid), [B] methanol
flow rate 1 mL/minsplit to the MS
sample glycitein, genistein at 1 µg/mL, equol at 50 µg/mL and daidzin, glycitin, genistin at 10 µg/mL in 60:40 10 mM ammonium formate (pH 3.0 with concentrated formic acid):methanol
injection 10 µl
detector MS, ESI(+) in selected ion recording (SIR) mode

Description

Categories Analytical Chromatography, Flavors, Fragrances
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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