HPLC Analysis of Isoflavones in Soy Milk on Ascentis® Express RP Amide (Milk Brand 1)

HPLC Analysis of Isoflavones in Soy Milk on Ascentis® Express RP Amide (Milk Brand 1)

Conditions

column Ascentis Express RP-Amide, 10 cm x 3 mm I.D., 2.7 µm particles (53918-U)
column temp. 30 °C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in methanol
gradient held at 15% B for 1 min; 15 to 100% in 5 min; held at 100% B for 1 min; 100-15% B in 0.1 min; held at 15% B for 2.9 min
flow rate 0.8 mL/min
pressure 2900 psi (200 bar)
sample soy milk, methanol extract (1. Measure 2 mL of each brand of soy milk (6 samples) 2. Add 8 mL of methanol and shake for 1 hour 3. Centrifuge tubes at 10000 rpm for 30 minutes 4. Transfer aliquot to autosampler vial)
injection 10 µL
detector UV, 260 nm

Description

Analysis Note This application demonstrates the separation of seven isoflavones on Ascentis Express RP Amide
Categories Analytical Chromatography, Food & Beverage Analysis, Hormones, Beverage Testing, Milk
Featured Industry Food and Beverages
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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