HPLC Analysis of Large DNA Fragments Containing More Than 10kb on TSKgel® DNA-STAT

HPLC Analysis of Large DNA Fragments Containing More Than 10kb on TSKgel® DNA-STAT

Conditions

column TSKgel DNA-STAT, 10 cm x 4.6 mm I.D., 5 µm particles (821962)
mobile phase [A] 0.02 M Tris-HCl, pH 8.5; [B] 1.0 M NaCl, pH 8.5
gradient 75 to 100% B in 20 min
flow rate 0.5 ml/min
sample 1 kbase ladder
detector UV, 260 nm

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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