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HPLC Analysis of Light Scattering Detection of BSA on Zenix® SEC-300

HPLC Analysis of Light Scattering Detection of BSA on Zenix® SEC-300

Conditions

column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles, 300 Ã… (Z777033)
column temp. 25 C
mobile phase 0.1M phosphate buffer
flow rate 1.0 ml/min
sample BSA (20mg/mL)
injection 20 µl
detector Hitachi-Elite LaChromL-2130 pump equipped with aµlti-angle light scattering detector (DAWN-HELIOS: Wyatt Technology),a refractive index detector (Hitachi L-2490) and a UV-Vis detector (Hitachi 2420)

Description

Analysis Note The chromatogram shows an overlay of the ultraviolet (UV), differential refractive index (RI) and µlti angle light scattering data (MALS) for a sample of bovine serum albumin; showing the prsenece of dimer and trimer as well as the monomer. The chromatogram illustrates the power of light scattering used with Sepax’s Zenix® SEC in protein characterization. The light scattering data makes it possible to determine the molecular mass for the monomer, dimer and trimer.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
Other Notes Acknowledgements and thanks to Matthew Thompson for this data.
Postdoctoral Researcher - Gianneschi Lab, Department of Chemistry & Biochemistry University of California, San Diego
suitability application for HPLC

Materials

     
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