HPLC Analysis of Lysozyme, Myoglobin and Impurities on Discovery® BIO Wide Pore C5

HPLC Analysis of Lysozyme, Myoglobin and Impurities on Discovery® BIO Wide Pore C5

Conditions

column Discovery BIO Wide Pore C5, 15cm x 4.6mm I.D., 5mum particles (568422-U)
column temp. ambient
mobile phase [A] (75:25) 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (25:75) 0.1% TFA in water:0.1% TFA in acetonitrile
gradient 0-100% B in 25 min
flow rate 1.0 ml/min
injection 12 µl in 0.1% TFA
detector UV, 220 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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