HPLC Analysis of mAb Charge Variants on TSKgel® CM-STAT

HPLC Analysis of mAb Charge Variants on TSKgel® CM-STAT

Conditions

column TSKgel CM-STAT, 10 cm x 4.6 mm I.D., 7 µm particles (821966)
column temp. ambient
mobile phase [A] 20 mM MES buffer, pH 6.0; [B] 0.5 M NaCl in buffer A, pH 6.0
gradient 0% B (0 min), 30% B (15 min), 100% B (15 min),0% B (17 min), 10% B (17 min), 10% B (21 min)
flow rate 1 mL/min
sample monoclonal antibodies (mAb A through E)
injection 20 µL
detector UV, 280 nm

Description

Analysis Note A TSKgel CM-STAT weak cation exchange (WCX) column was applied to separate charge variants of several monoclonal antibodies. The typical analysis time on conventional 25 cm long WCX columns of about eighty minutes could be significantly reduced when separation was performed on a 10 cm TSKgel CM-STAT column, filled with 7 μm particles. The analysis profiles for five antibodies show that high resolution analysis can be obtained in about 20 minutes analysis time.
Categories Analytical Chromatography, Antibodies, Peptides
Citation From "Ion Exchange Chromatography for the Characterization of Biotherapeutics" in The Supelco Reporter, Vol. 29.3, page 20.
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
suitability application for HPLC

Materials

     
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