HPLC Analysis of mAb on Antibodix® WCX, Effect of Particle Size (5 cm columns)

HPLC Analysis of mAb on Antibodix® WCX, Effect of Particle Size (5 cm columns)

Conditions

column Antibodix WCX-NP3 PEEK, 5 cm x 4.6 mm I.D., 3 µm particles (Z777288)
column Z777280 (Antibodix WCX-NP1.7, 5 cm x 4.6 mm, 1.7 µm particles)
column temp. ambient
mobile phase (A) 10 mM sodium phosphate buffer, pH 7.5; (B) A + 100 mM NaCl
gradient NP3 column: 15 to 55% B in 30 min, held for 3 min; NP1.7 column: 15 to 100% B in 60 min, held for 1.7 min
flow rate 0.5 ml/min for 3 min; 0.3 ml/min for 1.7 min
pressure 2180 psi (3 min), 3425 psi (1.7 min)
sample Monoclonal antibody, 5 mg/mL
injection 5 µl
detector UV, 214 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Antibodix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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