HPLC Analysis of Maté Leaves on Ascentis® Express C18

HPLC Analysis of Maté Leaves on Ascentis® Express C18

Conditions

sample/matrix UAE (Ultrasonic Extraction) maté extract (dried and minced leaves (7.5 g) from maté were extracted with 225 mL of methanol in an ultrasonic bath, thermostated at 75 +/- 0.5 C (potency: 90 W; frequency: 40 kHz))
column Ascentis Express C18, 150 x 4.6 mm I.D., 2.7 µm particles (53829-U)
column temp. 25 C
mobile phase [A] water, pH 3 with formic acid; [B] acetonitrile, pH 3 with formic acid
gradient 0 to 5% B in 20 min; 5 to 40% B in 30 min
flow rate 1 ml/min
injection 10 µl
detector PDA: range 215- 420 nm (8 µl detector cell volume, cell temp. 40 C, sampling rate 12.5 Hz, time constant 0.025 s)

Description

Categories Analytical Chromatography, Flavors, Fragrances
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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