HPLC Analysis of Membrane Protein Aquaporin-Z (AqpZ) on SRT® SEC-300, Octyl Glucoside Mobile Phase Additive

HPLC Analysis of Membrane Protein Aquaporin-Z (AqpZ) on SRT® SEC-300, Octyl Glucoside Mobile Phase Additive

Conditions

column SRT-SEC 300, 30 cm x 7.8mm I. D., 5 µm particles (Z777110)
mobile phase 20 mM tris-HCl pH 7.0, 190 mM NaCl, 10 mM KCl, 40 mM octyl glucoside
flow rate 1.0 ml/min
sample aqpZ, 6 mg/mL
injection 2 µl
detector UV, 280 nm

Description

Analysis Note SRT SEC-300 gave the best separation of the three columns tested. It exhibited fewer secondary interactions and provided baseline separation of the high molecular weight proteins.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information SRT is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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