HPLC Analysis of Methylphenobarbital (Mephobarbital) Enantiomers on Astec® CHIROBIOTIC® V

HPLC Analysis of Methylphenobarbital (Mephobarbital) Enantiomers on Astec® CHIROBIOTIC® V

Conditions

column CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 µm particles (11024AST)
column temp. 25 °C
mobile phase [A] ethanol; [B] hexane; (25:75, A:B)
flow rate 1 mL/min
sample 1 mg/mL in methanol
injection 10 µL
detector UV, 205 nm

Description

Analysis Note Methylphenobarbital (also known as mephobarbital and mephobarbitone) is a barbiturate derivative used primarily as an anticonvulsant, but also as a sedative and anxiolytic. It is a chiral compound and the enantiomers have been shown to exhibit different bioactivity, one narcotic, one convulsant, neither of which is seen in the racemate. Shown here is the rapid, efficient separation of methylphenobarbital enantiomers on an Astec CHIROBIOTIC V column. Highest grade HPLC solvents were used to supply low background interference and low particulate contamination for robust, trouble-free operation. A Sigma-Aldrich USP reference standard provided reliable identification and quantification.
Categories Analytical Chromatography, Pharmaceutical Analysis, Chiral Applications, Pharmaceutical (small molecule), Anxiolytics, Sedatives, Anticonvulsant/antiepiletic/antiseizure, Anxiolytics/Sedatives, All Pharmaceutical Applications
Featured Industry Pharmaceutical (small molecule)
Legal Information CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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