HPLC Analysis of Monoclonal Antibody Heavy and Light Chains on Zenix® SEC-300

HPLC Analysis of Monoclonal Antibody Heavy and Light Chains on Zenix® SEC-300

Conditions

column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles (Z777033)
mobile phase 0.1% TFA, 0.1% formic acid, 20% acetonitrile
flow rate 0.5 ml/min
sample Monoclonal antibody (1 mg/mL) reduced with 20 mM DTT
injection 20 µl
detector UV, 280nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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